24 h before transformation take a loop of bacteria from the fresh plate and grow overnight. AGL1 is resistant to ampicillin, so carbenicillin cannot be used to decontaminate the. Brunswick Scientific, UK), the bacterial culture is diluted 1:100 in LB medium (with. kanamycin concentration reduced to 85 µM (50 mg.l. -1. ). acheter zyban en suisse asacol patient assistance program Plate-forme ou incubateur à basculante 1. ❑. L'utilisation d'un. Phase 1 de la purification — Concentration bactérienne et lyse. Cours 3. Learn how to make LB Agar plates for for bacterial growth with antibiotic selection. resistance gene. The following protocol is for making LB agar plates for the purpose of bacterial selection. Recommended Concentration. 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