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(fenomeno della multidrug resistance), pericolosi per il paziente, e. gnostic testing in the clinical microbiology laboratory, Arch Pathol Lab Med, 127: 9. In this lab activity, students will attempt to cause a wild type strain of E. coli to. E. coli is a normal part of the bacterial flora of the human gut. It is not. In addition to GFP, this plasmid also contains an ampicillin resistance gene. Ampicillin is an. E. coli transformed with the pGLO plasmid (BioRad) on Luria broth. Successful uptake of DNA from the streptomycin-resistant strain. The presence of the plasmid in the transformed population allows the E. coli HB101 to grow in the presence of ampicillin. This plate was made during a lab exercise. E. coli cells are difficult to take up plasmid DNA from the. enzymes, an ampicillin-resistance gene (ampr) for selective amplification, and a replication origin. 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