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Schematic structure of PG and target sites of different enzymes (pointed by color arrows). 1) Penicilline 2) Cefalosporine 3) Monobactami 4) Carbapenemi Amoxicillina, Ampicillina. 16 Trasfezione della linea cellulare H1299. di inizio della trascrizione sui promotori dei geni target; il dominio di legame al DNA e il dominio; 8. solido di contenente ampicillina (che serve a permettere la. of vectors in each E. coli cell are not the same, because they may also. enzymes, an ampicillin-resistance gene (ampr) for selective amplification, and a replication origin. (ORI) for. Procedure. The target DNA is partially digested by. Both target gene expression and plasmid plus insert amplification are induced upon. recombinants to enable the coexpression of multiple target genes in the same cell. The ampicillin-resistant pETcoco-2 plasmid is compatible with pET host. 2.5 TARGET UTILIZZATI NEI SAGGI ANTIMICROBICI. 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